You can get as many tunes as you'd like. They can store on a computer and then drop into the autocal when ever you want too. The issue with an autocal is that it is locked to the tuners hardware and other tuners cannot tune for it unless it is unlocked from the original tuner. This can be done remotely and most don't charge for the service. Once it is unlinked, then it will be linked to the new tuners hardware and you will not be able to go back and use the original tuners tunes.
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